A molecular fingerprint technique to detect pollution-related changes in river cyanobacterial diversity

Humans now have a strong influence on almost every major aquatic ecosystem, and our activities have dramatically altered the quality of receiving waters worldwide. Thus, there is a continuous need to develop and apply novel and effective technologies to detect, manage, and correct human-induced degradation of aquatic systems. In the present work, we evaluated the molecular approach using polymerase chain reaction (PCR)-temperature gradient gel electrophoresis (TGGE) to measure changes in cyanobacterial diversity along a pollution gradient in a river and compared it with that of using microscopic observations of field-fixed and cultured samples. The different 16S rDNA genes present in the cyanobacterial community of each sampling point of the river were separated by TGGE, giving a characteristic pattern of bands for each site. This pattern represents a "fingerprint" of the community, allowing direct comparisons of the different samples. The TGGE results revealed that the structure of the cyanobacterial community differed along the pollution gradient of the river. Microscopic and molecular approaches showed that cyanobacterial diversity decreased in a downstream direction. Similar results were obtained by the two methods, as indicated by the high correlation between them. We suggest PCR-TGGE could be a useful and rapidly applied technique for the routine analysis of changes in cyanobacterial diversity in response to pollution, which would allow us to monitor rivers in surveillance networks of watercourse quality.     

Risk Analysis and Occurrence of Hepatitis E Virus (HEV) in Domestic Swine in Northeast Brazil

Anti-HEV antibodies were detected in animals from abattoir and in farms from northeast Brazil. Our results suggest that HEV is highly disseminated in the swine population and might present a great risk to animal handlers and for consumption of raw or undercooked meat and meat products in northeast Brazil.     

PRIVACY IN THE WORK PLACE: THE IMPACT OF BUILDING DESIGN

This study examines the impact of building design on privacy in two office environments at Gulfstream Aerospace Corporation, Inc., U.S.A. The findings offer a range of design resources that Facility Management at Gulfstream can utilize to manage privacy for their office workers. In a broader context, the theoretical considerations presented in this study, though still in their formative stage, offer additional insight into what office workers may think about privacy in the work environment. These theoretical considerations have far reaching implications for design professionals, and pending further research may prove cost effective.     

Quantifying the human health benefits of curbing air pollution in Shanghai

Urban development in the mega-cities of Asia has caused detrimental effects on the human health of its inhabitants through air pollution. However, averting these health damages by investing in clean energy and industrial technologies and measures can be expensive. Many cities do not have the capital to make such investments or may prefer to invest that capital elsewhere. In this article, we examine the city of Shanghai, China, and perform an illustrative cost/benefit analysis of air pollution control. Between 1995 and 2020 we expect that Shanghai will continue to grow rapidly. Increased demands for energy will cause increased use of fossil fuels and increased emissions of air pollutants. In this work, we examine emissions of particles smaller than 10 microm in diameter (PM10), which have been associated with inhalation health effects. We hypothesize the establishment of a new technology strategy for coal-fired power generation after 2010 and a new industrial coal-use policy. The health benefits of pollution reduction are compared with the investment costs for the new strategies. The study shows that the benefit-to-cost ratio is in the range of 1-5 for the power-sector initiative and 2-15 for the industrial-sector initiative. Thus, there appear to be considerable net benefits for these strategies, which could be very large depending on the valuation of health effects in China today and in the future. This study therefore provides economic grounds for supporting investments in air pollution control in developing cities like Shanghai.     

Incidence of stress in benthic communities along the U.S. Atlantic and Gulf of Mexico coasts within different ranges of sediment contamination from chemical mixtures

Synoptic data on concentrations of sediment-associated chemical contaminants and benthic macroinfaunal community structure were collected from 1,389 stations in estuaries along the U.S. Atlantic and Gulf of Mexico coasts as part of the nationwide Environmental Monitoring and Assessment Program (EMAP). These data were used to develop an empirical framework for evaluating risks of benthic community-level effects within different ranges of sediment contamination from mixtures of multiple chemicals present at varying concentrations. Sediment contamination was expressed as the mean ratio of individual chemical concentrations relative to corresponding sediment quality guidelines (SQGs), including Effects Range-Median (ERM) and Probable Effects Level (PEL) values. Benthic condition was assessed using diagnostic, multi-metric indices developed for each of three EMAP provinces (Virginian, Carolinian, and Louisianian). Cumulative percentages of stations with a degraded benthic community were plotted against ascending values of the mean ERM and PEL quotients. Based on the observed relationships, mean SQG quotients were divided into four ranges corresponding to either a low, moderate, high, or very high incidence of degraded benthic condition. Results showed that condition of the ambient benthic community provides a reliable and sensitive indicator for evaluating the biological significance of sediment-associated stressors. Mean SQG quotients marking the beginning of the contaminant range associated with the highest incidence of benthic impacts (73–100% of samples, depending on the province and type of SQG) were well below those linked to high risks of sediment toxicity as determined by short-term toxicity tests with single species. Measures of the ambient benthic community reflect the sensitivities of multiple species and life stages to persistent exposures under actual field conditions. Similar results were obtained with preliminary data from the west coast (Puget Sound).     

Canadian prairie rural communities: their vulnerabilities and adaptive capacities to drought

Droughts can have severe negative effects on the environment, society and economy. The drought of 2001–2002 caused severe strain on economic and social activities in western Canada, particularly on rural communities through changes in water resources. This paper examines physical and social vulnerabilities and associated adaptation measures undertaken and the adaptive capacity in communities in the South Saskatchewan River Basin, Canada. Although all of these communities were exposed to the 2001–2002 drought, they had different levels of impacts, resulting in different types of drought adaptation measures, some due to experience with previous droughts and some in response to the 2001–2002 drought. Communities with unreliable water supply were the most vulnerable to these droughts. This vulnerability resulted in historic adaptations being implemented (e.g., Hanna, Alberta) and re-active adaptations (e.g., Cabri, Saskatchewan). It is important to examine the effectiveness of the current adaptive strategies to cope with more extensive and extended drought situations. First Nation communities, such as the Kainai Blood Indian Reserve, have many social and environmental issues but the impacts from the drought were minor. The Reserve had implemented economic changes in the late 1980s to make it less vulnerable to drought but resulted in negative impacts to the Reserve’s social health. It is imperative to determine how vulnerable First Nation communities are and will to improve future adaptive capacity. This paper provides a snap shot view of how Canadian Prairie Communities have adapted to drought and how vulnerable they are to future drought situations.     

UVR-induced photoinhibition of summer marine phytoplankton communities from Patagonia

During austral summer 2006, experiments were carried out to evaluate the effects of ultraviolet radiation (UVR, 280–400 nm) on carbon fixation of natural phytoplankton assemblages from Patagonia (Argentina). Surface water samples were collected (ca. 100 m offshore) at mid morning using an acid-cleaned (1 N HCl) dark container. The short-term impact of UVR (measured as radiocarbon incorporation) was immediately assessed by exposing samples to three artificial illumination treatments: PAR (400–700 nm), PAR + UV-A (320–700 nm), and PAR + UV-A + UV-B (280–700 nm). Pico-nanoplankton characterized the assemblages, and taxon-specific pigment fingerprinting combined with CHEMTAX and supplemented with microscopic observations showed varied proportions of diatoms, chlorophytes, and cyanobacteria throughout January–February 2006. Photosynthetic efficiency, as assessed through assimilation numbers, was high [between 4.4 and 10.4 μg C (μg chl-a)⁻¹ h⁻¹], and it was probably favored by the supply of inorganic nutrients from the Chubut River. UVR-induced photoinhibition appeared to be related to the taxonomic composition: in general, higher photoinhibition was observed when diatoms dominated, whereas this was lower when samples were dominated by chlorophytes. Our data suggest that xanthophyll pigments might have provided only limited protection in these already highlighted acclimated assemblages.     

Organochlorine pesticides in feathers of three raptor species in southern Brazil

Environmental Science and Pollution Research - In this study, we determined the presence of organochlorine pesticides (OCPs) in back feathers from three raptor species, Phalcoboenus chimango,...